besA [2019-05-09 18:06:08]

Ferri-bacillibactin esterase

Locus: BSU32010

Isoelectric point: 8.20

Molecular weight: 35.34 kDa

Protein length: 289 aa

Gene length: 870 bp

Function: iron acquisition PubMed

Product: ferri-bacillibactin esterase

Essential: no

Synonyms: yuiI

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Homeostasis → Acquisition of iron → Acquisition of iron / Other
• Metabolism → Additional metabolic pathways → Iron metabolism → Acquisition of iron / Other
• Groups of genes → Efp-dependent proteins

Gene

Coordinates: 3,292,490 3,293,359

The protein

Catalyzed reaction/ biological activity

• catalyses ferri-bacillibactin hydrolysis leading to cytosolic iron release PubMed

Protein family

• Esterase D family (single member, according to UniProt)

Structure

• 2QM0 (from B. cereus, 46% identity)

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: besA
  Description: PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in Fur regulon
  • AbrB: repression, PubMed, in AbrB regulon

  Regulation

  • induced upon iron starvation (Fur) PubMed

  Additional information

  • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

Mutant

• MGNA-A650 (yuiI::erm), available at the NBRP B. subtilis, Japan
• BKE32010 (besA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCAGTACGACATTCCTC, downstream forward: _UP4_TGAGACATACTCAGCCTTGC
• BKK32010 (besA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCAGTACGACATTCCTC, downstream forward: _UP4_TGAGACATACTCAGCCTTGC

References

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